I downregulated one gene mRNA level using siRNA. Then interesting thing is the miRNA, which is located in this gene, expression level was also downregulated. But my previous experiments showed that the miRNA and the host gene use different promoter. So my explaination is that the siRNA downregulated miRNA by downregulating pri-miRNA. Does it make any sense? Please help me with this. I am so confused.
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If we somehow downregulate pri-miRNA, mature miRNA will also be downregulated?
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