3 replies to this topic

[sara.r]

Hi
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards

[Qundo12]

sara.r, on Mar 8 2010, 07:29 PM, said:

Hi
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards

The easiest way is trying the washing solution of other purification kit. I usually don't distinguish the one for the plasmid extraction kit and the one for the gel extraction kit, they are similar.

[tea-test]

I would says these washbuffers are just 70% ethanol.

[snoopyx]

tea-test, on Mar 8 2010, 10:33 AM, said:

I would says these washbuffers are just 70% ethanol.

You can check the Buffer composition here: http://openwetware.o...prep/Qiagen_kit