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plasmid extraction kit wash buffer solution


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3 replies to this topic

#1 sara.r

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Posted 08 March 2010 - 02:29 AM

Hi
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards

#2 Qundo12

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Posted 08 March 2010 - 05:10 AM

Hi
unfortunately I have lost my plasmid extraction kit solutions and now I have unused columns, I have the recipe for resuspension, lysis and neutralization solutions but I dont know the composition of wash buffer, does any body know how can I make it myself?
regards

The easiest way is trying the washing solution of other purification kit. I usually don't distinguish the one for the plasmid extraction kit and the one for the gel extraction kit, they are similar.

#3 tea-test

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Posted 08 March 2010 - 08:33 AM

I would says these washbuffers are just 70% ethanol.
tea-test: The artist formerly known as Ned Land

#4 snoopyx

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Posted 12 March 2010 - 10:59 AM

I would says these washbuffers are just 70% ethanol.


You can check the Buffer composition here: http://openwetware.o...prep/Qiagen_kit




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