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free bisulfite conversion kit


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#1 epigenius

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Posted 05 March 2010 - 01:22 PM

hey guys, saw this in Nature Methods:

www.epigentek.com/bisulflash

too bad im already finishing up on my methylation studies but it may be of interest to some of you

cheers

#2 MoB

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Posted 06 March 2010 - 02:45 AM

Okay, 10 reactions for free...

But this website borders on the impertinent. I think everybody knows who's behind 'Supplier Z' and 'Supplier Q'. And their benchmarks can only be reached in their dreams. I've recently tested and compared all available bisulfite-conversion kits and only two kits turned out to give high-conversion rates, high yields, high purity of the eluate and a low DNA-degradation. And these were the kits from 'Supplier Z' and Supplier Q'. Just to mention it: the bottom quality was reached by the Epigentek kit. Save your precious samples and don't use this kit!

#3 epigenius

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Posted 30 March 2010 - 08:40 AM

Okay, 10 reactions for free...

But this website borders on the impertinent. I think everybody knows who's behind 'Supplier Z' and 'Supplier Q'. And their benchmarks can only be reached in their dreams. I've recently tested and compared all available bisulfite-conversion kits and only two kits turned out to give high-conversion rates, high yields, high purity of the eluate and a low DNA-degradation. And these were the kits from 'Supplier Z' and Supplier Q'. Just to mention it: the bottom quality was reached by the Epigentek kit. Save your precious samples and don't use this kit!


Really? i don't know, I've had some pretty good experiences with an Epigentek kit (not for bisulfite conversion though). I started with Sigma's bisulfite and global DNA methylation quantitation kits until someone in these forums mentioned they were just re-badged Epigentek kits.

maybe I'm wrong -- but thanks for the info!




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