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Hematin


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#1 koljai

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Posted 05 March 2010 - 12:08 PM

Hi everyone,
I need help with weighting the hematin(um?)
I need to know how to weigh it so its concentration will bečíme 40µM?
Dimension is 20µ L.

Linear Formula:C34H33N4O5Fe
Molecular Weight:633.49

Thx!!!

:D

#2 newborn

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Posted 28 March 2010 - 11:27 PM

Hi everyone,
I need help with weighting the hematin(um?)
I need to know how to weigh it so its concentration will bečíme 40µM?
Dimension is 20µ L.

Linear Formula:C34H33N4O5Fe
Molecular Weight:633.49

Thx!!!

:P



-dissolve hematin in 20 mm NaOH or DMSO
-centrifugation for 10 min at 15,000 rpm
-remove pellet
hematin concentration is estimated from absorbance at 385 nm (εm m = 58,400) in 100 mm NaOH and adjusted to (Shaklai N., Shviro Y., Rabizadeh E., Kirschner-Zilber I.(1985) Biochim. Biophys. Acta 821:355–366)




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