After producing a protein with the TNT quick coupled transcription/translation system from Promega, is it possible to store the extract with the newly translated protein for a couple of days? I'm asking because if you only use a fraction of the translation reaction, and will be needing it again the next day (say for a pull-down or a gel shift, or whatever), it feels wrong to do a new reaction each day (and expensive).
I wonder if you can keep the extract at 4ºC or -20ºC without the protein losing its activity.
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laurequillo
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Posted 05 March 2010 - 12:44 PM
Ambrósio, on Mar 5 2010, 08:00 PM, said:
After producing a protein with the TNT quick coupled transcription/translation system from Promega, is it possible to store the extract with the newly translated protein for a couple of days? I'm asking because if you only use a fraction of the translation reaction, and will be needing it again the next day (say for a pull-down or a gel shift, or whatever), it feels wrong to do a new reaction each day (and expensive).
I wonder if you can keep the extract at 4ºC or -20ºC without the protein losing its activity.
I wonder if you can keep the extract at 4ºC or -20ºC without the protein losing its activity.
Why not at -80?
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