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Quantifying the degree of crosslinking


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#1 danish07

danish07

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Posted 05 March 2010 - 04:22 AM

Hi everyone,

I'm new to this forum but it appears to be a fantastic source of information with some great minds behind the scenes. I was hoping one of you might be able to help me out with a problem I've been having recently.

What I am looking for is a simple technique, not involving rare and expensive equipment, to quantify the degree of crosslinking within a sample of gelatin. At present I am looking at using Ninhydrin with a spetrophotometer (visible light) to quantify the degree of crosslinking by absorbance. Unfortunately though, I get the feeling that it may not be as sound in practise as in theory. If anyone has any suggestions as to an alternate technique, or a better way to do the ninhydrin assay, I'd be very grateful for their help.

In case I haven't mentioned enough; ninhydrin detects free amino groups --> crosslinking uses up these groups.

Thanks!
Danish




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