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Isolation of DNA from Scat


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#1 atulkathait

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Posted 04 March 2010 - 10:46 PM

Hi
I am trying to isolate the DNA from scats of vertebrates. I tried Phenol:Choloroform & GuSCN methods. but i am not able to get desired DNA. If I got somethings its not amplify in PCR properly and also some bacterial DNA is also there. So plz tell me how can i got the desired bateria free DNA from Scat

Edited by atulkathait, 04 March 2010 - 10:48 PM.


#2 HomeBrew

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Posted 05 March 2010 - 04:25 AM

We have had good results isolating bacterial DNA for PCR amplification from mouse and human feces using the ExtractMaster fecal DNA extraction kit from
Epicentre Biotechnologies.

#3 microgirl

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Posted 05 March 2010 - 07:18 AM

Try a Qiagen fecal kit. There's also MoBio kits - they generally will send you a couple preps for free to try them out - and they're good for environmental stuff.




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