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ELISA Question

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#1 anonymous



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Posted 14 November 2000 - 10:00 PM

Just a quick question someone might be able to help me with....I have a recombinant protein produced in a baculovirus system and a polyclonal sera that is produced to that purified recombinant protein. I suspect that some of the signal that I get in the ELISA is due to antibodies raised to co-purified baculovirus proteins. Can I use a similarly produced recombinant protein (which is not homologous to the protein of interest) to subtract out the signal that is seen from antibodies raised to co-purified baculovirus proteins, as long as the amounts of protein loaded are pretty much the same? Or is there a better way? Is there a specific calculation to help me with this subtraction, or if anyone knows of any references where this situation is addressed, I would be most grateful. Thank you in advance for your help. Ian

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