Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Difference between gentamycin and gentamycin sulphate


  • Please log in to reply
3 replies to this topic

#1 genom38

genom38

    member

  • Active Members
  • Pip
  • 13 posts
2
Neutral

Posted 04 March 2010 - 04:04 PM

Hi everybody,

I am a little confused about using antibiotics in cell culture and I need your help.

I am routinely working with MCF7 breast cancer cell line and up to now I was using gentamycin sulphate as antibiotic in the medium. Recently, it is finished and I could find "gentamycin" (not gentamycin sulphate). What I want to learn is:

1. Is there any difference between using gentamycin and gentamycin sulphate for culturing cells?
2. If there is difference, should I perform a cytotoxicity test for gentamycin (new antibiotic) before I use it?

I need your immediate help, thank you in advance...

#2 rhombus

rhombus

    Rhombus/Uncle Rhombus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 214 posts
20
Excellent

Posted 05 March 2010 - 03:53 AM

Hi everybody,

I am a little confused about using antibiotics in cell culture and I need your help.

I am routinely working with MCF7 breast cancer cell line and up to now I was using gentamycin sulphate as antibiotic in the medium. Recently, it is finished and I could find "gentamycin" (not gentamycin sulphate). What I want to learn is:

1. Is there any difference between using gentamycin and gentamycin sulphate for culturing cells?
2. If there is difference, should I perform a cytotoxicity test for gentamycin (new antibiotic) before I use it?

I need your immediate help, thank you in advance...



MCF-7 is a common cell line we use in our Institute. The answer to your question is:-

YOU DON'T NEED TO USE ANTIBIOTICS/ANTIMYCOTICS IN THE FIRST PLACE.

All such compounds are very "Dirty"....i.e. they can interfer with many cellular mechanisms. You get the cells from the ATCC/ECACC free from Mycoplasma and adventisious agents. If you have good technique then they are not required.

Kindest regards.

Uncle Rhombus.

#3 genom38

genom38

    member

  • Active Members
  • Pip
  • 13 posts
2
Neutral

Posted 06 March 2010 - 02:02 PM

Hi everybody,

I am a little confused about using antibiotics in cell culture and I need your help.

I am routinely working with MCF7 breast cancer cell line and up to now I was using gentamycin sulphate as antibiotic in the medium. Recently, it is finished and I could find "gentamycin" (not gentamycin sulphate). What I want to learn is:

1. Is there any difference between using gentamycin and gentamycin sulphate for culturing cells?
2. If there is difference, should I perform a cytotoxicity test for gentamycin (new antibiotic) before I use it?

I need your immediate help, thank you in advance...



MCF-7 is a common cell line we use in our Institute. The answer to your question is:-

YOU DON'T NEED TO USE ANTIBIOTICS/ANTIMYCOTICS IN THE FIRST PLACE.

All such compounds are very "Dirty"....i.e. they can interfer with many cellular mechanisms. You get the cells from the ATCC/ECACC free from Mycoplasma and adventisious agents. If you have good technique then they are not required.

Kindest regards.

Uncle Rhombus.


Hi Rhombus,

Thank you for your answer. I have been using medium w/o antibiotic for a while, however we are so many people working in the same lab (in cell culture room). I have tried to develop a resistant mcf7 against a chemotherapeutic agent for over 1 year and my cell lines are so sensitive and important. Namely, I am anxious about my resistant cell lines because of not having enough stock. Hence, I am planning to start to use antibiotic soon. If you know the difference between gentamycin and gentamycin sulphate, would you please help me about this?

#4 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
7
Neutral

Posted 06 March 2010 - 08:15 PM

Hi,

Here's a link to Drug information at Merk.

regards,

lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.