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Ethanol in PCR clean-up


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3 replies to this topic

#1 phosphate girl

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Posted 04 March 2010 - 04:48 AM

Hello,

I am new to this forum and molecular biology generally. So I have been doing PCR, I now need to clean-up my product and am using a Min-Elute kit from Qiagen. I have to dilute the buffers included with ethanol of which we have lab stocks. My dumb question: Is it safe to use these lab stocks ? How susceptible to contamination is ethanol? I will soon be moving onto RNA work and am also wondering the same thing......can I trust lab stocks of ethanol or should I buy in my own........can I make ethanol nuclease free??

Thanks

Oh am using the PCR product for sequencing after clean-up

Edited by phosphate girl, 04 March 2010 - 04:49 AM.


#2 phage434

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Posted 04 March 2010 - 07:01 AM

Assuming your lab is not populated by high school students, I would use the ethanol without worry.

#3 hanming86

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Posted 04 March 2010 - 08:11 AM

just make sure the ethanol is at least 95% pure.

i have seen Industrial based ethanol (Cheap stufff) that we would use for flaming or the typical 70% etoh disinfectant being used for DNA-based work.

urg needless to say inconsistent sequencing result, bad digest etc etc.

it will smell different. pure ethanol won't sting the nose as compared to cheap one
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#4 phosphate girl

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Posted 08 March 2010 - 04:02 AM

Hello guys!

Thanks so much for your replies - it's nice just to get a bit of reassurance from somebody!

Hope your work is going OK and better than mine

:)




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