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jurkat transfections of TCR


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#1 anonymous

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Posted 15 January 2001 - 10:00 PM

I am thinking of getting a Jurkat cell line that doesn't express its vBeta chain and thus is CD3-negative. I was wondering if someone can assist how best to transfect this cell line with the cDNA of the desired vBeta chain and which would be the best promoter to use in this procedure. Thanks for any insight, Shirin




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