I wanna consult every master-hands here with a problem I had just met in previous days. I picked up my only one colony transformed bacterium DH5a on the Amp(+) LB-agar plate and to transfer it to Amp(+) LB liquid for to grow the cell at 37'C 255rpm overnight, but the DH5a didn't grows in the Amp(+) LB liquid in Falcon Tube, it still not grow after I change new LB liquid(pH7.4), so I suppose the concentration of the antibiotic was too high, so I do it again today as that to change the concentration from
9ul Amp.(100mg/ml) per 9ml LB liquid to
8ul Amp.(100mg/ml) per 9ml LB liquid, but I don't know whether is it right or not?
Edited by learner2010, 03 March 2010 - 02:04 PM.