Jump to content


- - - - -

protein extraction from tissue, help


1 reply to this topic

#1 Zhe

    member

  • Active Members
  • Pip
  • 5 posts

Posted 03 March 2010 - 08:58 AM

Hi there,

I need to extract proteins from a tiny muscle fibre. I'm tending to use the T-PER tissue protein extraction reagent. But I don't know how i could perform the homogenization. Obviously, a hand-hold glass homogenizer won't do since my muscle tissue is very small. Any suggestion on this plz? like sonication?

Many thx,
Jo

#2 mdfenko

    an elder

  • Active Members
  • PipPipPipPipPip
  • 1,790 posts

Posted 03 March 2010 - 09:13 AM

in what volume will you be extracting? there are some very small teflon-glass homogenizers.

you could even homogenize in a eppendorf tube.
talent does what it can
genius does what it must
i do what i get paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.