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protein extraction from tissue, help


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#1 Zhe

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Posted 03 March 2010 - 08:58 AM

Hi there,

I need to extract proteins from a tiny muscle fibre. I'm tending to use the T-PER tissue protein extraction reagent. But I don't know how i could perform the homogenization. Obviously, a hand-hold glass homogenizer won't do since my muscle tissue is very small. Any suggestion on this plz? like sonication?

Many thx,
Jo

#2 mdfenko

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Posted 03 March 2010 - 09:13 AM

in what volume will you be extracting? there are some very small teflon-glass homogenizers.

you could even homogenize in a eppendorf tube.
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