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Protein extraction from tissue


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#1 Zhe

Zhe

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Posted 03 March 2010 - 08:40 AM

Hi there,

I need to extract proteins from a tiny muscle fibre. I'm tending to use the T-PER tissue protein extraction reagent.  But I don't know how i could perform the homogenization. Obviously, a hand-hold glass homogenizer won't do since my muscle tissue is very small. Any suggestion on this plz? like sonication?

Many thx,
Jo

#2 bob1

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Posted 03 March 2010 - 03:55 PM

you can get small (0.5 ml) glass homogenisers from Kontes glassware?, if that is any help.

Edited to add link to 0.5 ml Dounce.

Edited by bob1, 03 March 2010 - 04:05 PM.





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