Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Relative RT-PCR


  • Please log in to reply
2 replies to this topic

#1 alirezakhodavandi

alirezakhodavandi

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 03 March 2010 - 05:59 AM

Dear all
I have to analyze gene expression between 2 genes and actin as a house-keeping gene at 6 time intervals in plants using from one step RT-PCR kit. My problem now, is the number of amplification cycles, because for optimization of these genes 40 cycle was better, I want to know for relative RT-PCR this number of amplification cycle is sufficient or no?
best regards

#2 susanna

susanna

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 79 posts
0
Neutral

Posted 04 March 2010 - 02:02 AM

40 to 50 cycles are ok

#3 alirezakhodavandi

alirezakhodavandi

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 04 March 2010 - 06:16 AM

40 to 50 cycles are ok


Thanks for reply




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.