Dear all
Actually, I'm not expert in real time PCR, and want to know the role of standard curve in relative real time RT-PCR.
Thanks and best regards
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#2
ivanbio
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Posted 03 March 2010 - 07:28 AM
The main purpose of a standard curve in real time PCR is to determine if your assay is working as it should. If your standard curve does not give you an r2 >0.99 and an efficiency between 90 and 110%, then there is something wrong with your assay and you should find a way to make it better or re-design it.
Ivan
Carlsbad, CA
#3
alirezakhodavandi
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Posted 04 March 2010 - 06:28 AM
ivanbio, on Mar 3 2010, 06:58 PM, said:
The main purpose of a standard curve in real time PCR is to determine if your assay is working as it should. If your standard curve does not give you an r2 >0.99 and an efficiency between 90 and 110%, then there is something wrong with your assay and you should find a way to make it better or re-design it.
