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40 pcr cycles and Ct-values of 36-40 - trust or not?


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#1 wozzels

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Posted 03 March 2010 - 02:04 AM

Hey all,

I was wondering, if you run 40 pcr cycles, are Ct-vales between 36-40 meningful or not?
I thought that Ct-values, close to the number of cycles, should be examined with great caution.
And can somebody give me any references (scientific articles) dealing with these topics?

Thank you in advance!
Wozzels

#2 susanna

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Posted 04 March 2010 - 01:54 AM

hi,

If you're doing double tests, and both replica have a Cp of 36, i would still use these values, especially if you see that you also get a high Cp-value, using a housekeeping gene on the same sample. In contrast, i would not use Cp-values of 39, 40.


Hey all,

I was wondering, if you run 40 pcr cycles, are Ct-vales between 36-40 meningful or not?
I thought that Ct-values, close to the number of cycles, should be examined with great caution.
And can somebody give me any references (scientific articles) dealing with these topics?

Thank you in advance!
Wozzels






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