Electric field and ionic strength effects!!!
Posted 03 March 2010 - 01:15 AM
P.S> I use 500 mM NaOH and 500 mM Ortho Phosphoric acid as catholyte and anolyte. I will be trying a 25 mM solutions to check if buffer ionic strength has a role to play in the current fluctuations at higher voltages.
Posted 03 March 2010 - 08:43 AM
if you are seeing current fluctuation at high voltage then you are probably experiencing arcing. you may have a dry spot or a crack in your gel.
reducing the ionic strength of your electrolytes may make matters worse. you should not change the tried and true procedure (especially if you are using prepared gels or a kit).
inspect your gel prior to and during the run to see if there is a flaw where arcing can occur or is occurring.
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 04 March 2010 - 02:01 AM
i tried doing a step voltage increase with time so as to control the current... seems to have worked.. thanx again!!
Edited by Pradeep Iyer, 04 March 2010 - 02:02 AM.