hi i have a question, suppose i make a set of primers- one forward and one reverse.. on making the primer when i do blast i find that one primer- say left primer is binding only at one locus in homo sapies but the other primer is binding at different chromosomes, so will the overall product size vary, or not.
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#2
pcrman
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Posted 02 March 2010 - 06:43 PM
most likely you won't get any product if the two primers are not on the same chromosome or are far away from each other.
#3
zuda
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Posted 02 March 2010 - 06:54 PM
NO THAT IS NOT THE CASE, THE PRIMERS ARE ON THE SAME CHROMOSOME , BUT ALSO ONE OF THEM IS FOUND AT ANOTHER CHROMOSOME
#4
Vini
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Posted 02 March 2010 - 08:06 PM
zuda, on Mar 3 2010, 09:24 AM, said:
NO THAT IS NOT THE CASE, THE PRIMERS ARE ON THE SAME CHROMOSOME , BUT ALSO ONE OF THEM IS FOUND AT ANOTHER CHROMOSOME
in that case, u will get the product with primers on the same chromosome....not the other one....
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pcrman
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Posted 02 March 2010 - 08:27 PM
I agree. the mismatch won't be a problem as long as the other does not has mismatch.
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Posted 04 March 2010 - 01:57 AM
you only have amplication, when both primers bind your product. The same thing using an extra probe to increase specificity
pcrman, on Mar 2 2010, 09:27 PM, said:
I agree. the mismatch won't be a problem as long as the other does not has mismatch.
