Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

peritoneal fluid dilution


  • Please log in to reply
3 replies to this topic

#1 ujla80

ujla80

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 70 posts
0
Neutral

Posted 02 March 2010 - 07:27 AM

hi all :P

i had to collect the peritoneal fluid from normal control mice, but since it was difficult to collect the fluid from live mice, i injected 2 ml of normal saline into the cavity and inturn i got 1 ml of peritoneal fluid (from the test mice with peritoneal inflammation it is not difficult to collect the peritoneal fluid). i did NO estimation in the fluid by a biochemical method. now i am getting problem in calculation...what would be the dilution factor for the sample?

please help!!! :)
god is great

#2 Gerard

Gerard

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts
0
Neutral

Posted 02 March 2010 - 02:14 PM

I would apply no dilution factor, you can't compare your control mice direct with the test mouse because you cant tell'how the extra volume production off the fluid influence the results. Just write exactly in your publication under methods what has been done.

I have another concern, if you are working with animals prepare your experiments very carefully. I think you behavior is ethically not very decent when you have to repeat the experiment because you didn't think it over very carefully.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 ujla80

ujla80

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 70 posts
0
Neutral

Posted 03 March 2010 - 09:07 AM

thank you Gerard for the answer...i didn't know it is ethically wrong to take out peritoneal fluid from the live mice..now i'll collect the fluid at the time of dissection after sacrificing it by cervical dislocation...then i may not need to inject saline in the cavity and would know approx amount of fluid in the cavity...but if i don't use the dil. factor would my data be accepted for the publication?






I would apply no dilution factor, you can't compare your control mice direct with the test mouse because you cant tell'how the extra volume production off the fluid influence the results. Just write exactly in your publication under methods what has been done.

I have another concern, if you are working with animals prepare your experiments very carefully. I think you behavior is ethically not very decent when you have to repeat the experiment because you didn't think it over very carefully.


god is great

#4 sgt4boston

sgt4boston

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 276 posts
1
Neutral

Posted 03 March 2010 - 12:54 PM

As long as you handle both types of animals in the same fashion. Flush the cavities of both with saline. The volume of fluid is probably not the major item of interest...it is probably the protein or agent that is in the fluid. I imagine you have to purify or quantify the 'contents' of the fluid be it cells, antibody etc.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.