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Increasing Separation of Bands on SDS PAGE gel


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#1 LeneHarman

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Posted 02 March 2010 - 01:02 AM

Hello,

Does anybody have any suggestions as to how I could increase the separation of two bands running ~50 and ~52kDa on a SDS PAGE gel? These bands are partially glycosylated states of a receptor subunit. At the moment the two bands partially merge together. I have run the samples on a 7.5% and on a 10% acrylamide gel but with the same result.

Thanks,

Lene

#2 K.B.

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Posted 02 March 2010 - 02:14 AM

Have you tried 12% or 15% gels? Bands will be higher but may separate. Also I think Tris-Tricine PAGE can give you bit better resolution.

#3 ursae

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Posted 18 July 2010 - 06:06 PM

have u tried running at a very slow speed using a high % gel for maybe 2-3hrs? Cos....50 & 52kda is too close.




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