2 replies to this topic

[LeneHarman]

Hello,

Does anybody have any suggestions as to how I could increase the separation of two bands running ~50 and ~52kDa on a SDS PAGE gel? These bands are partially glycosylated states of a receptor subunit.  At the moment the two bands partially merge together.  I have run the samples on a 7.5% and on a 10% acrylamide gel but with the same result.

Thanks,

Lene

[K.B.]

Have you tried 12% or 15% gels? Bands will be higher but may separate. Also I think Tris-Tricine PAGE can give you bit better resolution.

[ursae]

have u tried running at a very slow speed using a high % gel for maybe 2-3hrs?  Cos....50 & 52kda is too close.