Increasing Separation of Bands on SDS PAGE gel
Posted 02 March 2010 - 01:02 AM
Does anybody have any suggestions as to how I could increase the separation of two bands running ~50 and ~52kDa on a SDS PAGE gel? These bands are partially glycosylated states of a receptor subunit. At the moment the two bands partially merge together. I have run the samples on a 7.5% and on a 10% acrylamide gel but with the same result.
Posted 02 March 2010 - 02:14 AM
Posted 18 July 2010 - 06:06 PM