Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

IHC vs qPCR


  • Please log in to reply
1 reply to this topic

#1 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 196 posts
2
Neutral

Posted 01 March 2010 - 11:53 PM

Hi,

I need to check the relationship between 2 genes. The problem is that there is good antibody for one gene but the second gene does not a have good commercially available antibody. Is it possble to compare the IHC staining results of one gene with that of qPCR expression data of the second gene? This may not be the best approach but has anyone published data this way?



Thank you

#2 scolix

scolix

    a student

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 314 posts
5
Neutral

Posted 02 March 2010 - 11:49 AM

IHC and qpcr look at different things. qPCR-RNA and IHC - protein.

Think about what you need for your experiment.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.