How to culture cells after sorting?
Posted 01 March 2010 - 07:54 AM
Posted 06 March 2010 - 07:10 AM
How are cells cultured after subjecting them to antibodies and cell sorting? How are the bound antibodies removed after sorting? What are the preparation steps after the cells are collected?
Posted 08 March 2010 - 09:00 AM
Posted 11 March 2010 - 05:54 PM
Hi, thanks for the reply. I will be using different markers to sort 4T1 mouse breast cancer cell line then culture the cells and propagate them afterwards. Is there any other treatment steps that I need to do after sorting? What do you do in your lab? Are the bound antibodies causing any problems?