Posted 01 March 2010 - 04:46 AM
Any ideas? Perhaps the sonication time was excessive?
Posted 01 March 2010 - 09:22 AM
Posted 01 March 2010 - 10:31 AM
Posted 02 March 2010 - 02:49 AM
I was trying two different buffers, one was something I have always used (tris, DTT, EDTA and NaCl at pH 8), the other was bought from Qiagen. But I didn't get a pellet for either once I had spun them down!
I've never had this problem before but I guess I will soon find out if this really matters soon enough!