Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

measuring apoptosis

  • Please log in to reply
1 reply to this topic

#1 SF_HK



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 206 posts

Posted 28 February 2010 - 08:05 PM


My gene of interest results in reduced growth rate compared to non-transfced parental cell line. Furthermore, transient transfection of my gene resukts in enhanced cleaved PARP.

I want to study if teh reduced cell proliferation is due to apoptpsis, what would be teh best way to approach this?

I'm thinking of using Caspase Inhibitor Z-VAD-FMK. Has anyone used this? Also, this kit inhibits caspas but the user has to carry out standard apoptosis assay to measure the level of aopotosis. I;m not not what approach to take?

Any ideas would be welcomed.

#2 scolix


    a student

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 314 posts

Posted 01 March 2010 - 10:19 AM

you are right, an apoptosis assay kit would do. you could look at different stages of apoptosis also. There are different assays kits for these also.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.