Designing primers with ESTs
Posted 27 February 2010 - 04:52 PM
I have decided to design primers using EST sequences since the species (sus scrofa) I am involved with have less mRNA data in Genebank. I am doing RT-PCR for my microarray validation. What are the pit falls and risks of designing primers using ESTs. Can I cite them like mRNA sequences? Please let me know what you all think. thanks in advance.
Posted 27 February 2010 - 06:37 PM
Posted 28 February 2010 - 12:41 AM
Thanks for the reply. appreciated.