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spot removal from 2D gel and proteomics


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#1 rijomarmi

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Posted 26 February 2010 - 08:11 PM

I need to remove spots from my 2D gels for proteomics. I stain my gels with syproruby. Can I leave the gels in sypro ruby overnight at 4C and do the spot removal in the next day, or should I remove the spots immediately after staining?
thanks!

#2 fishdoc

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Posted 27 February 2010 - 09:02 AM

I need to remove spots from my 2D gels for proteomics. I stain my gels with syproruby. Can I leave the gels in sypro ruby overnight at 4C and do the spot removal in the next day, or should I remove the spots immediately after staining?
thanks!




Overnight is fine. I've left gels stained with Sypro Ruby for 6 months with no substantial loss of signal.




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