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qPCR Standard Deviation Calculations and Data Representation


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#1 Gold Blend

Gold Blend

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Posted 26 February 2010 - 04:42 PM

I have a question related to qPCR data standard deviation calculation. When presenting my data in column form, I take the negative reciprocal of fold change values that are less than one (Fold change of 0.5 become -2). A problem arises when I attempt to calculate the standard deviation of replicate sets with fold changes greater and less than one. As an example, the following set of fold changes (0.50, 0.67, 0.57) I would convert to (-2, -1.5, -1.75) and calculate the standard deviation as I am treating the entire set equally. If I have a set with the following (2.0, 1.5, 0.5) I feel like I cannot just convert the 0.5 to -2 and take the standard deviation of the three as I would be treating one member of the set differently than the others. How do I go about calculating and presenting the data in this case? This would not be a problem if I just reported the less than one fold changes as is, but this makes the data difficult to represent on a graph - should I just use a table instead? Any help would be appreciated.

-Adam




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