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Program for determination of kinetic parameters of an enzyme


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#1 sebinou

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Posted 26 February 2010 - 12:51 PM

Hi,

I've generated improved mutants of my favorite enzyme, and now I need to determine their kinetic parameters (specially Vmax). I've obtained a gross estimation using Lineweaver-Burke plots made with Excel. But I read that, although such linear representations are useful for visualizing data, they should not be used to determine kinetic parameters, as computer software is readily available for more accurate determination by nonlinear regression methods.

Can anybody recommend a software (preferably free) to carry out such analyses ?

Thank you

#2 Felipillo

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Posted 27 February 2010 - 08:13 PM

Check Dynafit, it's free under academic license

http://www.biokin.com/dynafit/

You can do a simulation your data, and propose a kinetic mechanism.

Edited by Felipillo, 27 February 2010 - 08:14 PM.

Chance favors the prepared mind
Louis Pasteur.

#3 sebinou

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Posted 28 February 2010 - 10:19 AM

Thank you, i'm gonna try it !




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