2 replies to this topic

[sebinou]

Hi,

I've generated improved mutants of my favorite enzyme, and now I need to determine their kinetic parameters (specially Vmax). I've obtained a gross estimation using Lineweaver-Burke plots made with Excel. But I read that, although such linear representations are useful for visualizing data, they should not be used to determine kinetic parameters, as computer software is readily available for more accurate determination by nonlinear regression methods.

Can anybody recommend a software (preferably free) to carry out such analyses ?

Thank you !

[Inmost sun]

sebinou, on Feb 26 2010, 09:49 PM, said:

Hi,

I've generated improved mutants of my favorite enzyme, and now I need to determine their kinetic parameters (specially Vmax). I've obtained a gross estimation using Lineweaver-Burke plots made with Excel. But I read that, although such linear representations are useful for visualizing data, they should not be used to determine kinetic parameters, as computer software is readily available for more accurate determination by nonlinear regression methods.

Can anybody recommend a software (preferably free) to carry out such analyses ?

Thank you !

try Grafit (a free trail version is available)

Grafit

I think Sigmaplot is also to use...

[CAT]

En.. free software,. you may try graphpad demo version. Origin is good too. I just do not know whether there is free origin.