Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Probe manufacturers, who makes best probes?


  • Please log in to reply
3 replies to this topic

#1 patty4150

patty4150

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 25 February 2010 - 01:12 PM

I have gotten some FAM/BHQ probes from a vendor and although they work initially, they go off quickly (within weeks.) The primers (from another source) seem fine.

I do not freeze thaw repeatedly, I protect from light, and I keep them stored at -80 long term. I am thinking the synthesis is not good. It has been a problem with all the probes we have ordered from this company.

Where do you get your real-time PCR probes, and have they been stable?

Edited by patty4150, 25 February 2010 - 01:12 PM.


#2 ivanbio

ivanbio

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 116 posts
7
Neutral

Posted 25 February 2010 - 03:21 PM

Hi patty,

Assuming that your problem is the probes and not some other issue, a vendor I've used extensively for qPCR probes is Eurogentec. I've had probes last close to 2 years without any noticeable loss in signal. I never freeze and thaw more than once; I prepare a bunch of aliquots and store them frozen until I use them.

Ivan
Carlsbad, CA

#3 pcrmossad

pcrmossad

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 27 February 2010 - 04:59 PM

I have gotten some FAM/BHQ probes from a vendor and although they work initially, they go off quickly (within weeks.) The primers (from another source) seem fine.

I do not freeze thaw repeatedly, I protect from light, and I keep them stored at -80 long term. I am thinking the synthesis is not good. It has been a problem with all the probes we have ordered from this company.

Where do you get your real-time PCR probes, and have they been stable?



I buy them from thermo electron. If you aliquote them and store them at -20 , theiy are fairly stable, and even worked after one year.

http://www.thermo.co...ome/1,,,00.html

#4 patty4150

patty4150

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 03 March 2010 - 08:07 PM

I have gotten some FAM/BHQ probes from a vendor and although they work initially, they go off quickly (within weeks.) The primers (from another source) seem fine.

I do not freeze thaw repeatedly, I protect from light, and I keep them stored at -80 long term. I am thinking the synthesis is not good. It has been a problem with all the probes we have ordered from this company.

Where do you get your real-time PCR probes, and have they been stable?



I buy them from thermo electron. If you aliquote them and store them at -20 , theiy are fairly stable, and even worked after one year.

http://www.thermo.co...ome/1,,,00.html

thank you both. I am trying Biosearch next, and hoping for a better stability than seen with the previous manufacturer.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.