Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Different values of the same sample in ELISA


  • Please log in to reply
1 reply to this topic

#1 Crispinheiro

Crispinheiro

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 25 February 2010 - 09:01 AM

Hi,

Does anybody know how can I use the results of two different plates in an ELISA test. I immunized mice with my recombinant protein and I used ELISA to detect IgG antibodies againt my recombinant protein. For this assay, I had to use two plates and I put a positive control serum in both plates. The O.D. of one plate (control serum) was 0,72 and the other one was 0,85. How can I use the results of the two plates, considerating that they have different O.D of the control serum. Is there a method for correcting the value of the positive control of different plates?

Thanks in adavnce!

Best regards,

Cris

#2 sgt4boston

sgt4boston

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 276 posts
1
Neutral

Posted 25 February 2010 - 10:55 AM

You would have had to run 2-3 points of the dose response curve on the second plate. Then you would have been able to adjust the values based on any shift observed in the curve.

Alternatively, if you ran replicates of any of the samples which have values over a wide range of the curve you could do a comparison plot and adjust the values based on slope and intercept.

can others comment?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.