
Lysis buffer
#1
Posted 25 February 2010 - 06:27 AM
I'm trying to prepare 10X lysis buffer, but I have problems with solubility. The buffer should be 0,5 M EDTA; 0,5 M Tris; and 10 % SDS. I adjusted pH just above 8, but there is something that doesn't want to dissolve. Could you please help me!?
#2
Posted 25 February 2010 - 08:17 AM
"This is SPARTA!"
"I´m the goddamn batman"
#3
Posted 25 February 2010 - 08:21 AM
#4
Posted 25 February 2010 - 11:02 PM
#5
Posted 25 February 2010 - 11:45 PM
Yes, I tried also to warm it to 70-80 C... It didn't help. There solution is otherwise clear, but there is a thick precipitation at the top...
Why don you try to add the SDS to the final 1X solution? I know that a 1%SDS solution is ok, I never tried such amount of SDS.
"This is SPARTA!"
"I´m the goddamn batman"
#6
Posted 26 February 2010 - 12:27 AM
#7
Posted 26 February 2010 - 06:45 PM
Your life would be much simpler if you made a 5x buffer, using 0.5 M EDTA stock solution, 1 M Tris solution, and 20% SDS.
#8
Posted 01 March 2010 - 06:29 AM

Thank you all for your comments, finally after a week in + 70 C my buffer seems to be clear!
#9
Posted 01 March 2010 - 03:30 PM
#10
Posted 04 March 2010 - 08:25 AM
But why should life be simple and without challenges
Thank you all for your comments, finally after a week in + 70 C my buffer seems to be clear!
I also suspect it is EDTA. A week in 70C.. crazy haha.
however if at all time u could keep it simple, please do. Research is a race against time.