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[S_M]

Hi All,

I'm new here. I've just been overexpressing a pre-miR in a cell line. Surprising'y, when I'd assay for the mature miRNA I was seeing a downregulation (compared to baseline control levels)...I subsequently realized I was actually assaying the miR* (anti-sense) strand, i.e. the strand that's degraded and not incorporated into the RISC. I've subsequently confirmed overexpression using an assay for the sense strand. However, I was wondering why, when assaying for the antisense strand, I was seeing levels lower than that in the control. What could be the reason for this. Could the cells be over-zealous in their attempt to downregulate all the exogenous antisense strands, actually reducing levels to below baseline? Would just appreciate some thoughts on this...

thanks,

SM