4 replies to this topic

[BryanC]

Hello All,

New to the forum, but have gotten great info in the past when searching for something on google.  


I have a heparin stock solution at 1000 U/ml.  Is it possible to make a 50mg/ml working stock from this solution?

If so, what other info do i need for the calculation?

Thanks,
BC

[almost a doctor]

BryanC, on Feb 24 2010, 03:26 PM, said:

Hello All,

New to the forum, but have gotten great info in the past when searching for something on google.  


I have a heparin stock solution at 1000 U/ml.  Is it possible to make a 50mg/ml working stock from this solution?

If so, what other info do i need for the calculation?

Thanks,
BC

You need to know how many U are in a mg, and calculate from there

[BryanC]

Thanks.


So, if the Heparin salt is 180 U/mg, then the 1000 U/ml sol'n is at .180 mg/ml?  Which means I would have to make a fresh 50 mg/ml stock from the heparin salt?

Guess Ill have to order some more heparin, because someone has put a basically empty bottle back into the cabinet without ordering more.  

Edited by BryanC, 24 February 2010 - 01:22 PM.

[bob1]

180 U/mg will give 1000U.ml^-1/180U.mg^-1= 5.556 mg/ml not 0.18mg/ml - you'll still have to make a fresh stock or add a larger volume to a dilution.

[BryanC]

bob1, on Feb 24 2010, 06:08 PM, said:

180 U/mg will give 1000U.ml^-1/180U.mg^-1= 5.556 mg/ml not 0.18mg/ml - you'll still have to make a fresh stock or add a larger volume to a dilution.

Thanks bob.  I really rushed that calc.  And man was I  over thinking U/ml, U/mg, etc.  Never had to deal with "units" before....  

Being that it's for in situ hyb, i don't think I trust the cleanliness of someone else's solution anyway.