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RNA isolation with peqGold TriFast


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#1 rephi

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Posted 24 February 2010 - 02:42 AM

Hi!

I want to extract total RNA from bacteria from environmental samples. During my search for an easy and appropriate protocol I came across the peqGold Trifast solution. Does anyone have experience with this? Does it produce good results?

Thank you!
rephi

#2 tea-test

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Posted 25 February 2010 - 01:23 AM

Hello,

I'm using it for isolating RNA from mammalian cells and tissues and it works quite good. Before, I used Trizol from Invitrogen, but I have the impression that Trifast gives a more stable interphase after chloroform centrifugation. The removing of the upper aqueous phase is much faster and easier. Concerning purity and yield I would say they are comparable.
tea-test: The artist formerly known as Ned Land

#3 rephi

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Posted 01 March 2010 - 02:36 AM

Thank you! I will try it out myself and post about my experiences later on..




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