2 replies to this topic

[rephi]

Hi!

I want to extract total RNA from bacteria from environmental samples. During my search for an easy and appropriate protocol I came across the peqGold Trifast solution. Does anyone have experience with this? Does it produce good results?

Thank you!
rephi

[tea-test]

Hello,

I'm using it for isolating RNA from mammalian cells and tissues and it works quite good. Before, I used Trizol from Invitrogen, but I have the impression that Trifast gives a more stable interphase after chloroform centrifugation. The removing of the upper aqueous phase is much faster and easier. Concerning purity and yield I would say they are comparable.

[rephi]

Thank you! I will try it out myself and  post about my experiences later on..