RNA isolation with peqGold TriFast
Posted 24 February 2010 - 02:42 AM
I want to extract total RNA from bacteria from environmental samples. During my search for an easy and appropriate protocol I came across the peqGold Trifast solution. Does anyone have experience with this? Does it produce good results?
Posted 25 February 2010 - 01:23 AM
I'm using it for isolating RNA from mammalian cells and tissues and it works quite good. Before, I used Trizol from Invitrogen, but I have the impression that Trifast gives a more stable interphase after chloroform centrifugation. The removing of the upper aqueous phase is much faster and easier. Concerning purity and yield I would say they are comparable.
Posted 01 March 2010 - 02:36 AM