2 replies to this topic

[Crispinheiro]

Hello,

I have two recombinant proteins that are expressed as inclusion bodies and when I try to purify these inclusion bodies I obtain many contaminants. I have been using  a buffer composed by sodium phosphate 20 mM, NaCl 500mM and Imidazole 10 mM, followed by lisozyme (1mg/mL) and sonication, in bacterial lysis process. Then I use the same lysis buffer + Urea 8M to denaturate my protein. Does anybody know how can I obatin inclusion bodies with high purity?
Thanks very much!

[KAUSHIK THAKKAR]

Please go through the attached paper (highlighted text) for purification of protein from IBs.


Hope this helps.

Best regards,
Kaushik

Attached Files

•  Paper_for_IB_purification.pdf   1.21MB   350 downloads

[Crispinheiro]

KAUSHIK THAKKAR, on Feb 24 2010, 06:12 AM, said:

Please go through the attached paper (highlighted text) for purification of protein from IBs.


Hope this helps.

Best regards,
Kaushik

Dear Kaushik,

Thank you very much. I will try this protocol.

best wishes,

Cris