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Inclusion Body purification


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#1 Crispinheiro

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Posted 23 February 2010 - 05:45 PM

Hello,

I have two recombinant proteins that are expressed as inclusion bodies and when I try to purify these inclusion bodies I obtain many contaminants. I have been using a buffer composed by sodium phosphate 20 mM, NaCl 500mM and Imidazole 10 mM, followed by lisozyme (1mg/mL) and sonication, in bacterial lysis process. Then I use the same lysis buffer + Urea 8M to denaturate my protein. Does anybody know how can I obatin inclusion bodies with high purity?
Thanks very much!

#2 KAUSHIK THAKKAR

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Posted 24 February 2010 - 01:12 AM

Please go through the attached paper (highlighted text) for purification of protein from IBs.
:P

Hope this helps.

Best regards,
Kaushik
:(

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#3 Crispinheiro

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Posted 24 February 2010 - 05:18 AM

Please go through the attached paper (highlighted text) for purification of protein from IBs.
:P

Hope this helps.

Best regards,
Kaushik
:(



Dear Kaushik,

Thank you very much. I will try this protocol.

best wishes,

Cris




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