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[little friend]

Hi,

I would like to subclone a promoter (spaghetti squash in drosophila) that I have in an expression vector into a transformation vector (pUASP). The pUASP vector has UAS binding site and transposase promoter downstream of the UAS binding site. There is a HindIII site 5' of the UAS binding site and KpnI site 3' of the transposase promoter (both unique sites). So I am wondering if I could just PCR amplify the spaghetti squash promoter and subclone it into the pUASP vector via the HindIII and KpnI sites. Are there other considerations that I need to take into account? Like the length of the linking region between promoter and transgene, 3' UTR, etc? Cutting out the UAS binding site will leave 13bp of the 5' UAS binding sequence behind which will be situated right upstream of the spaghetti squash promoter. Will this affect promoter function?

Any help/suggestions will be appreciated.

Thanks!

Little friend