1 reply to this topic

[Signal]

Hi all,

I am now proceeding to western blot analysis of the protein under my investigation. I have no experience of protein work so any lead or help regarding antibody design/softwares etc would be highly appreciated.....

cheers

[bob1]

Antibody production is expensive, unless you can't find any commercial antibodies (most companies have an antibody finding service that also covers other companies) for your protein, I would go with commercial antibodies.

If not - read "Antibodies : a laboratory manual" Ed Harlow, David Lane.  1988.  It is a good starting manual.