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How to design an antibody


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#1 Signal

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Posted 23 February 2010 - 07:51 AM

Hi all,

I am now proceeding to western blot analysis of the protein under my investigation. I have no experience of protein work so any lead or help regarding antibody design/softwares etc would be highly appreciated.....

cheers

#2 bob1

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Posted 24 February 2010 - 05:50 PM

Antibody production is expensive, unless you can't find any commercial antibodies (most companies have an antibody finding service that also covers other companies) for your protein, I would go with commercial antibodies.

If not - read "Antibodies : a laboratory manual" Ed Harlow, David Lane. 1988. It is a good starting manual.




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