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Membrane Solubilize with CHAPSO


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#1 alexanc2

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Posted 23 February 2010 - 07:43 AM

Hi everyone,

In my lab we solubilize membrane with 1% CHAPSO and then use the centrifuge to separate out the insoluble from the soluble fractions. What would I expect to find in the insoluble fraction and how do I study this?

Thank you

#2 mdfenko

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Posted 23 February 2010 - 11:14 AM

membrane fragments, insoluble proteins and nuclei (and maybe some other organelles).

Edited by mdfenko, 23 February 2010 - 11:14 AM.

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