Pipette tips for gel electrophoresis?
Posted 23 February 2010 - 06:36 AM
I got a question regarding on loading PCR products into gel.
I have been using filter tips for all PCR preps (from preparing master mix to PCR to gel electrophoresis)
and would like to lower the expenses as filter tips r more expensive than ordinary pipette tips
I am wondering whether would it be alright to use ordinary pipette tips to load PCR prodcuts into the agarose gel seeing that there will be no amplifications taking place during gel electrophoresis. Would it contaminate my PCR products? I usually keep my PCR products in the freezer -20C just as a precaution if I needed to re-gel.
Im relatively new to PCR, thus any advices are greatly appreciated.
Sorry if this question has been asked
Posted 23 February 2010 - 07:46 AM
Posted 23 February 2010 - 08:09 AM
Posted 23 February 2010 - 05:31 PM
Did not know that I could use ordinary tips for all steps~this will definitely lower my cost
Thanks a lot!
Posted 24 February 2010 - 03:11 PM