i too have recently been getting what i think is a leak but after the gel polymerizes there is a thin 1mm space at the bottom.
i have never had a problem making gels and they usually solidify in at most 15 minutes. Yesterday i spent 5 hours remaking gels because they would either leak out of the sandwich or not solidify properly (there are imperfections in the gel which resembled different parts of the gel solidifying at different times).
i am making an 8% gel how long does it usually take to harden?
my APS was only 2 weeks old would it make a huge difference? i also have been ejecting the TEMED down the side of the tube instead of mixing it directly.
i threw out a gel yesterday because i saw the 1mm space at the bottom and after pouring on the stack gel i noticed that the stack gel level fell and i couldn't help thinking it it leaked through my resolving gel. i did noticed new gel formation at the bottom where the space was.
i also mix by turning it upside down and i don't degas...are these potential problems? i mena i've never experienced anything til recently. i started suspecting the brand new bottle of acrylamide:bis solution so i borrowed the neighboring lab's. i think it's working better? i think i've lost all confidence
Edited by azrael201, 17 February 2011 - 12:13 PM.