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How to concentrate my protein?
Started by kfszelok, Feb 21 2010 07:08 PM
1 reply to this topic
#1
Posted 21 February 2010 - 07:08 PM
I am new in protein work.
I am purifying scretory protein in the growth medium. However, it is too diluted after quantification. What is the easiest way to concnetrate the protein (say if I want to use 100ml broth?)? Many Thanks!

#2
Posted 22 February 2010 - 01:51 AM
It depends on what materials and equipment do you have in your lab.
- ultrafiltration
- freeze-drying (lyophilization)
- precipitation (acetone, ethanol, methanol, ammonium sulfate)
- ultrafiltration
- freeze-drying (lyophilization)
- precipitation (acetone, ethanol, methanol, ammonium sulfate)