I am purifying scretory protein in the growth medium. However, it is too diluted after quantification. What is the easiest way to concnetrate the protein (say if I want to use 100ml broth?)? Many Thanks!
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#1
kfszelok
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Posted 21 February 2010 - 07:08 PM
I am new in protein work. I am purifying scretory protein in the growth medium. However, it is too diluted after quantification. What is the easiest way to concnetrate the protein (say if I want to use 100ml broth?)? Many Thanks!
I am purifying scretory protein in the growth medium. However, it is too diluted after quantification. What is the easiest way to concnetrate the protein (say if I want to use 100ml broth?)? Many Thanks!
#2
K.B.
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Posted 22 February 2010 - 01:51 AM
It depends on what materials and equipment do you have in your lab.
- ultrafiltration
- freeze-drying (lyophilization)
- precipitation (acetone, ethanol, methanol, ammonium sulfate)
- ultrafiltration
- freeze-drying (lyophilization)
- precipitation (acetone, ethanol, methanol, ammonium sulfate)
