storing RNA at -20C?
Posted 21 February 2010 - 02:23 PM
how long can RNA be stored at -20 C?
alternatively i could drive it over to another facility with an -80 C freezer - but that would be like 30 minutes in a car. any methods of lowering the temperature while transporting it? dry ice?
Posted 21 February 2010 - 02:28 PM
Posted 21 February 2010 - 03:39 PM
Posted 22 February 2010 - 02:18 PM
Posted 22 February 2010 - 02:39 PM
you can also store RNA in salt and ethanol until you go back to your lab and then precipitate it for your microarray experiments. RNA in ethanol is stable even at RT for considerably longer than in water. Its quite easy than cheaper than spending much on matrix like RNAstable if you have large number of samples.RNAstable is good...I've tried RNAstable for a month or so and ethanol for over 2 weeks at RT and RNA was still intact...if ethanol does the same for half the period at almost no cost, why bother?!!
Dry ice is the best way for long transport, but as you say its 30 min, you can as well do that in a -20 mini cooler or even normal ice!