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[blaszcz]

I want to stain RNA in 8M urea denaturated polyacrylamide gel. I must remove urea from gel prior to stain beacuse urea react with silver. Do you know how can I remove it? I found only one information that I should incubate gel in 1xTBE buffer for 15min before staining

any other tips?

best regards

blaszcz

[mdfenko]

soaking is the way to remove urea from the gel. you were instructed to soak in tbe, we soak in fixative (methanol/acetic acid). you can use a relatively large volume once or can use less more than once.