does anyone know the concentration of salt and primers the methprimer software uses to calculate primer Tm? Because I ordered the primers I got from the software and I had put it at 60°C Tm. However on the datasheet of eurogentec it gives me Tm's of about 40°C. And if I fill it in in a primer Tm calculator it still gives me ridiculously low temperature with the concentration given on the kit!
Edited by Spliffeh, 19 February 2010 - 05:22 AM.