question about sequncing
Posted 18 February 2010 - 10:09 PM
my question is
what is the meaning of hieght of peak, sometime it is short and sometime it is long for same nucleotide.
plz answer any one
Posted 19 February 2010 - 09:11 AM
genius does what it must
i do what i get paid to do
Posted 24 February 2010 - 01:10 PM
*When the sequence is not very clean but we suspect that there is a real base pair change, we compare the dosage amount of the suspicious peak to that of the WT or control. If the dosage drops by half, the change is probably real. There's a program called Sequence Pilot that saves the peak height information and you can compare the normal peak heights to the one you're looking at.