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How to do PCR to detect mRNA without RT?


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#1 bluesoul

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Posted 18 February 2010 - 09:17 PM

Hi all,

My experiment is going to detect whether some gene's mRNA level is changed or not after some treatment. Is it possible to detect mRNA level without RT using PCR? If it is possible, what's the potential benefit doing this way?

Thanks.

HB.

#2 leelee

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Posted 18 February 2010 - 09:42 PM

No, it's not possible.

#3 bluesoul

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Posted 19 February 2010 - 07:08 AM

Thanks.

Have a nice day.


No, it's not possible.






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