I have expressed his-tagged protein which is of about 53KDa. But it is showing about 65KDa size on SDS-PAGE. Recombinant protein is also reacting with specific polyclonal sera. Why there is size descrepency i dont know. May be Histidine 6X tag influences the mobility of protein in SDS-PAGE. Can any body help me out.
Edited by Dr Rakesh Kumar, 18 February 2010 - 05:15 AM.