Hello all!
I am triying to develop a Ni chromatography. I have the idea that you should use a pH of 8 to have your his-tag in positive charge (as the pKa of His is nearly 7, you have to take a lot of care with pH). I am using as buffer for diluting my seed, Borax 20 mM pH 10, i assume that once you are over the His pKa, the used pH is irrelevant. Any of you have a clear idea about this issue?
Thank you in advance
0
No replies to this topic
#1
paramyosin
-
- Active Members
-
- 34 posts
Enthusiast
0
Neutral
Neutral
Posted 18 February 2010 - 03:48 AM
"Research without indebtedness is suspect, and somebody must always, somehow, be thanked." Umberto Eco
